We get to choose 10 plays to practice for the game at the end of the week. My question is...... If we do not have a play checked, but the red bar under is all the way up, do we still get that familiarity bonus when that play is called during the game, Or must we have that play checked to get the familiarity bonus no matter what the red bar is?

As I understand it, you get the familiarity bonus whether or not you study it for that week. If you study it you get another boost on top of that.